Twodimensional 2d immunoelectrophoresis, also known as crossed immunoelectrophoresis, is a particularly useful technique for the quantitation of mixtures of proteins and the analysis of the. Insulatorbased dielectrophoretic manipulation of dna in a microfluidic device by lin gan a dissertation presented in partial fulfillment of the requirements for the degree doctor of philosophy approved july 2015 by the graduate supervisory committee. Certified that the thesis entitled study on the effect of air space. Pohl 23 manipulated yeasts saccharomyces cerevisiae thanks to a nonuniform electric field induced by the alternative current polarization of two metallic electrodes. The 50% discount is offered for all ebooks and ejournals purchased on igi globals online bookstore. Recommended citation pandian babu, vandana devi, problems in the study and use of ac dielectrophoresis and. Provision of arsenic free water is the only option to safeguard public health in. Twodimensional crossed immunoelectrophoresis springer. In this technique sodium dodecyl sulfate acts as a dissociating agent in order to. The certified thesis is available in the institute archives and special collections. A portable and integrated instrument for cell manipulation. Clinically, it is often desirable to isolate cell subpopulati. Dielectrophoresis is similar but uses a shaped ac field to determine the dielectric properties.
In this thesis a parametric optimization is used to determine the optimum radius of the post for particle separation. I also wish to thank sari nieminen, joni hattuniemi and matti saren who gave me the spark for research and with whom i had many enlightening and supportive conversations during the work as well as in our free time. This thesis is brought to you for free and open access by the theses at tigerprints. The nonuniform electric field is formed by applying ac voltage between a pin electrode and a plate electrode.
However, the strength of the force depends strongly on the. Electrophoresis, as its name suggests, measures the electric properties of a material that is, its net charge. Design and fabrication of novel 3d dielectrophoresis cell. Collectively these magnetic imaging studies deepen our knowledge of. This force does not require the particle to be charged. The digital version of your thesis must be submitted in pdf format. Ebooks and ejournals are hosted on igi globals infosci platform and available for pdf andor epub download on a perpetual or subscription basis. Ultimately the thesis was a result of the strong collaborative efforts between both supervisors. It has been accepted for inclusion in all theses by an authorized administrator of tigerprints. Ultrasound fluid and cell manipulation diva portal.
Assessment of changes in the dielectric properties of multidrug resistant cancer cells by electrorotation technique submitted by garsha bahrieh in partial fulfillment of the requirements for the degree of master of science in electrical and electronics engineering department, middle east technical university by, prof. Mike arnold of industrial research, for his helps and practical advices that have improved my work tremendously. The increasing trend toward quality assurance programs and hazard analysis, consumer demand for a wide variety of wholesome foods, and legislative pressures e. We do this by observing particle motion at several ac frequencies, rather than at dc, and inferring the properties from. Dielectrophoresis dep has been reported by several laboratories as a promising method in biomedical research for label. Fabrication of a 3 dimensional dielectrophoresis electrode. Sandias dielectrophoresis device may revolutionize sample. Manipulation and analysis of microparticles in a microfluidic device finds wide application in numerous fields such as application in microbiology, drug and medicine assessment, pointofcare for disease diagnosis and microengineering.
Subramanyan namboodiri varanakkottu geboren in mandur, indien. To initiate the free download, click on the full text pdf link that appears underneath the citation and abstract information of the dissertation. Comprehensive coverage of the basic theoretical concepts and applications of dielectrophoresis from a worldrenowned expert. Based on theory, simulations were performed, resulting in six different labonachips are designed that have been manufactured. Cell separation is an important component of modern medicine, with both clinical and research applications. This thesis presents the design, fabrication, and implementation of er devices with 3d electrodes for the dielectric characterization of cells. William wells became the first director of the school of engineering, and by 1987, all eligible. Evidence from china, phd thesis, department of accountancy, city university of hong kong, hong kong, prc, 4 may 2015. In addition, as an experimental application of the extracted dielectric properties of the cells by er, the dep method is utilized. Several label free optical techniques include spr9 and raman spectroscopy10 have been.
Different microfluidic devices were designed and developed to meet the needs for every application based on different engineering practices. The uid dynamics involved and the dielectric properties of water inside singlewalled carbon nanotube are studied as well. Polarization and manipulation at liquidliquid interfaces by mitchell desmond a thesis submitted to johns hopkins university in conformity with the requirements for the degree of master of science engineering baltimore, maryland august, 2014. Fabrication of a 3 dimensional dielectrophoresis electrode by. The steps described below also cover cosupervised thesis. Selectivity is defined as the degree to which a method is free from interference by other species contained in the sample matrix 1. Selecting this option will search all publications across the scitation platform selecting this option will search all publications for the publishersociety in context. Quinn, carmel maria 1995 dielectrophoresis of microorganisms treated with antimicrobial agents. Board of regents approved separate bachelors and masters degrees in mechanical engineering, civil engineering, electrical engineering, and computer science. These affect the particles dipole, and allow us to determine the dielectric properties such as resistance and capacitance. Clinically, it is often desirable to isolate cell subpopulations providing focused treatment. Web to pdf convert any web pages to highquality pdf files while retaining page layout, images, text and. Darteurope is a partnership of research libraries and library consortia who are working together to improve global. This discount cannot be combined with any other discount or promotional offer.
The electrodes are composed of bottom planar gold au electrodes and three dimensional 3d silver ag electrodes fabricated locally on the au electrode through metal inkjet printing. This collection of mit theses in dspace contains selected theses and. Theses and dissertations, free to find, free to use. An ids system usually consist of a rf frequency voltage source up to 24 v, syringe pumps, microscope, and computer. Alexandra ros, chair daniel buttry yan liu arizona state university august 2015. Bioparticle capture and sensing utilizing nanograss based microfluidic devices by hengkan ni thesis submitted in partial fulfillment of the requirements for the degree of master of science in electrical and computer engineering in the graduate college of the university of illinois at urbanachampaign, 2012 urbana, illinois adviser. Gel electrophoresis is widely used in forensics, molecular biology, genetics, and biotechnology. Dielectrophoresis dep and electrowetting ewod as antifouling processes for antibacterial surfaces. Download fulltext pdf dielectrophoresis dep and electrowetting ewod as antifouling processes for antibacterial surfaces thesis pdf available june 2014 with 147 reads. Experimental development and analysis of a novel setup for.
Results and discussion platform design and development. Modeling of dielectrophoresis in micro and nano systems. Graduate thesis or dissertation from electrophoresis to. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Cores of the study lie on the microelectrode designs that are capable of. Our cell densitometry platform, magdense, is composed of i two permanent magnets 50mm length, 2mm width, and 5mm height with the same poles.
This thesis presents the development of a label free cell discriminator for dengueinfected cells using dielectrophoresis dep. We observed the negative dep characteristics of the 4. The microvalves are specifically designed for labonachip applications to achieve leakage ratios below 0. The versatility and the uniqueness of dep makes it an attractive tool, especially in. Of particular interest was the application of dep to viruses, the largest of which is approximately 250 nm in diameter. Dielectrophoresisbased spherical particle rotation in 3d. Experimental development and analysis of a novel setup for insulated dielectrophoresis a thesis presented to the graduate school of clemson university in partial fulfillment of the requirements for the degree master of science bioengineering by. Dielectrophoresis of submicrometre particles enlighten.
Twodimensional 2d immunoelectrophoresis, also known as crossed immunoelectrophoresis, is a particularly useful technique for the quantitation of mixtures of proteins and the analysis of the composition of protein mixtures. A system for virus characterisation, identification and separation based. This electronic version was submitted by the student author. Dep force depends on the size and the electrical properties of the particles and the suspending medium. Dielectrophoresis an overview sciencedirect topics.
Cells in the reproductive state, such as murine sarcoma ascites cells and rapidly growing murine l fibroblasts, selectively attract highly polarizable small particles. This file is licensed under the creative commons attributionshare alike 4. Dielectrophoresis dep is a phenomenon in which a force is exerted on a dielectric particle when it is subjected to a nonuniform electric field. Dep is applicable even for nonconducting particles and can be. For this purpose, two different microvalves are presented in the study. Use pdf download to do whatever you like with pdf files on the web and regain control.
Abstract of the thesis high efficiency threedimensional manipulation of submicron particles and dna molecules by alexander leroy teeter master of science in mechanical engineering san diego state university, 2006 this thesis theoretically quantifies the different forces appropriate for controlled. The research described in this thesis was focused on the possibility to separate motile and nonmotile spermatozoa and to separate viable and nonviable spermatozoa onchip with a technique called dielectrophoresis. Figure 1 illustrates the behaviors of particles in the nonuniform electric field. Insulatorbased dielectrophoretic manipulation of dna in a microfluidic device by lin gan a dissertation presented in partial fulfillment of the requirements for the degree. Bachelor of sciences in engineering mechanical engineering. Dielectrophoresis is an electrokinetic phenomenon acting on polarizable particles in an inhomogeneous electric field. The dielectrophoresis group at the university of surrey was established in 1999. Onchip separation of spermatozoa using dielectrophoresis. It has produced nearly 80 papers in the field of dielectophoresis and related phenomena, three books and six patents and have been cited over 5500 times. We proposed a micro electrode fabrication method by a metal inkjet printing technology for the bioapplications of dielectrophoresis dep.
I would like to express my gratitude to the people who involves in this thesis. The integrity of any chemical measurement depends on a mechanism to selectively interrogate the desired component in a mixture. It uses a dc voltage to attract anions and cations to the relevant counterelectrode, and the speed and direction of moveme. The aim of this phd project was to develop the technology of dielectrophoresis on the submicrometre scale and to use dep to manipulate submicrometre particles and measure their dielectric properties. This thesis presents an active microarray that exploits electrokinetic electrophoresis and dielectrophoresis forces for its hybridization method using 3d carbon electrodes that will enable the large volume manipulation for pathogen detection. It also acts as a link between the university and the community, as it demonstrates the excellent research results that can benefit the development of the region.
A portable and integrated instrument for cell manipulation by. Our cell densitometry platform, magdense, is composed of i two permanent magnets 50mm length, 2mm width, and 5. When the dielectric constant of particle is larger than that of. Our expertise is in the development of lowcost, robust technology for dielectrophoresis appications, and in. I have a question regarding the solver while solving the dielectrophoresis. Novel distributed lubrication system to improve the. Modeling of dielectrophoresis in micro and nano systems yuan lin 2008 kth mechanics se100 44 stockholm, sweden abstract this thesis presents models and simulations of dielectrophoretic separation of micro and nano particles. It can be used to characterise, manipulate and separate these particles. This website is built in order to fulfill the requirement of the course sqg 3163 techniques in molecular biology under the supervision of dr salehhuddin bin hamdan. Jun 29, 2010 this option allows users to search by publication, volume and page selecting this option will search the current publication in context. It has been widely used to analyze nucleic acids and proteins.
This thesis presents analysis, design, implementation, and testing of electrostatically actuated mems microvalves. All particles exhibit dielectrophoretic activity in the presence of electric fields. On having consulted this thesis youre accepting the following use conditions. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses.
Dielectrophoresis is defined as the motion of a neutral particle caused by polarization effects in a nonuniform electric field1,2. Design and simulation of a microscale magnetophoretic device. Design and simulation of a microscale magnetophoretic. There are several programs that can produce such a pdf file. Dep, the induced movement of dielectric particles in a nonuniform electric field, has been used as a potential technique for the manipulation and separation of many biological samples without destructive.
Diagnostics, cellbased drug discovery, sensors for biomedical applications, characterisation and sorting of stem cells, separation of cancer cells from blood and environmental monitoring. Electrokinetic biosensing at liquidliquid interfaces. Ultrasonic fluid and cell manipulation mathias ohlin doctoral thesis department of applied physics kth royal institute of technology stockholm, sweden, 2015. The design and evaluation of microelectrode patterns on a. This option allows users to search by publication, volume and page selecting this option will search the current publication in context. Iso dielectrophoresis ids can be used to characterize and separate cells by their electrical characteristics. Dep is a phenomenon related to electrophoresis, but with some important differences.
Dielectrophoresis of microorganisms treated with antimicrobial agents. To define the effective moment, it is convenient to start with the electrostatic potential due to this electric dipole 1. Insulatorbased dielectrophoretic manipulation of dna in a. Pdf dielectrophoresis dep and electrowetting ewod as. Electrokinetic biosensing at liquidliquid interfaces by nicholas mavrogiannis a thesis submitted to johns hopkins university in conformity with the requirements for the degree of master of science in engineering baltimore, maryland. A crosscountry analysis, phd thesis, department of accountancy, city university of hong kong, hong kong, prc, 4 december 2014. This report summarizes the research outputs of our research degree and professional doctorate students, and serves to recognize their hard work and perseverance. The radboud university thesis repository university library. Pdf on the dielectrophoretic particle retention in. The metaphase plate is removed using a blunt enucleation pipette right and a separation needle left. Novel distributed lubrication system to improve the excessive. Microdielectrophoresis of dividing cells springerlink.
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